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SAB4200106

Sigma-Aldrich

Anti-ULK1 antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonym(e):

Anti-ATG1 autophagy related 1 homolog, Anti-UNC51, Anti-Unc51.1, Anti-unc-51-like kinase 1

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~150 kDa

Speziesreaktivität

human

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

Konzentration

~1.0 mg/mL

Methode(n)

western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human ULK1.

UniProt-Hinterlegungsnummer

O75385

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ULK1(8408)

Verwandte Kategorien

Allgemeine Beschreibung

Uncoordinated 51-like kinase 1 (ULK1) is expressed in skeletal muscle, heart, pancreas, brain, placenta, liver, kidney and lung. This gene is located on human chromosome 2q24.3. ULK1 localizes to cytoplasmic structures. ULK1 and ULK2 is a homolog of autophagy related 1 (Atg1) gene.

Spezifität

Anti-ULK1 recognizes human ULK1.

Anwendung

Anti-ULK1 has been used in immunoblotting.

Biochem./physiol. Wirkung

Uncoordinated 51-like kinase 1 (ULK1) is involved in modulating Atg9 subcellular dynamics. Knockdown of ULK1, but not ULK2, inhibits the autophagic response. Overexpression of ULK1 also inhibits autophagy, suggesting that ULK1 may have multiple mechanisms for regulating autophagy. Knockdown of Ulk1 shRNA can prevent gastric cancer cell survival.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Ulk1 over-expression in human gastric cancer is correlated with patients' T classification and cancer relapse
Chen MB, et al.
Testing, 8(20), 33704-33704 (2017)
Gemcitabine induces poly (ADP-ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer
Wang Y, et al.
PLoS ONE, 9(10), e109076-e109076 (2014)
siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy
Chan EYW, et al.
The Journal of Biological Chemistry, 282(35), 25464-25474 (2007)
Human ULK1, a Novel Serine/Threonine Kinase Related to UNC-51 Kinase ofCaenorhabditis elegans: cDNA Cloning, Expression, and Chromosomal Assignment
Kuroyanagi H, et al.
Genomics, 51(1), 76-85 (1998)
Xin Yu et al.
Journal of molecular neuroscience : MN, 50(3), 586-599 (2013-04-18)
Spinocerebellar ataxia type 7 (SCA7) is one of nine neurodegenerative disorders caused by expanded polyglutamine domains. These so-called polyglutamine (polyQ) diseases are all characterized by aggregation. Reducing the level of aggregating polyQ proteins via pharmacological activation of autophagy has been
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