S1629
Sulfatase aus Aerobacter aerogenes
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
Synonym(e):
Arylsulfatase
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About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54
Empfohlene Produkte
Typ
Type VI
Qualitätsniveau
Form
buffered aqueous glycerol solution
Spezifische Aktivität
2-5 units/mg protein (biuret)
Mol-Gew.
~41 kDa
Konzentration
10-20 units/mL
Fremdaktivität
β-Glucuronidase ≤10 U/mL
Versandbedingung
wet ice
Lagertemp.
−20°C
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Allgemeine Beschreibung
Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.
Anwendung
Sulfatase from Aerobacter aerogenes has been used:
- as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
- in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
- to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides
Biochem./physiol. Wirkung
Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.
Einheitendefinition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.
Physikalische Form
Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
Substrat
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
T Saidha et al.
Archives of biochemistry and biophysics, 272(1), 237-244 (1989-07-01)
Mitochondria that have been purified from cells of light-grown wild-type Euglena gracilis Klebs var. bacillaris Cori or dark-grown mutant W10BSmL and incubated with 35SO4(2-) and ATP accumulate a labeled compound in the surrounding medium. This compound is also labeled when
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
Bioluminescent probes of sulfatase activity.
Jason S Rush et al.
Chembiochem : a European journal of chemical biology, 11(15), 2096-2099 (2010-09-28)
Toyokazu Miura et al.
Bioscience, biotechnology, and biochemistry, 70(6), 1509-1512 (2006-06-24)
Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme
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