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N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

liquid

Synonym(e):

pNPP

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About This Item

Lineare Formel:
O2NC6H4OP(O)(ONa)2
CAS-Nummer:
4264-83-9
Molekulargewicht:
263.05
MDL-Nummer:
MFCD00007319
UNSPSC-Code:
12352204
PubChem Substanz-ID:
24897835
NACRES:
NA.83

Form

liquid

Qualitätsniveau

200

Versandbedingung

wet ice

Lagertemp.

−20°C

SMILES String

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChIKey

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Verwandte Kategorien

Allgemeine Beschreibung

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Anwendung

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem./physiol. Wirkung

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Vorsicht

Stable at least one year when stored below 0 °C.

Physikalische Form

Ready-to-use.

Piktogramme

CorrosionHealth hazard
GHS05,GHS08

Signalwort

Danger

Gefahreneinstufungen

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Lagerklassenschlüssel

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen® Custom Peptide Library

Review a high-throughput ELISA-based method to identify peptide substrates for class-specific and/or enzyme-specific Protein Tyrosine Kinases (PTKs) that can be utilized for the detection of kinase activity both in vivo and in vitro.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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