Monoclonal Anti-Myosin Light Chain Kinase antibody produced in mouse

Monoclonal Anti-Myosin Light Chain Kinase (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin light chain kinase (MLCK) is a calcium/calmodulin-dependent enzyme (approx.160 kD). It is expressed in skeletal, cardiac, smooth and mammalian non-muscle cells. MLCK is encoded by the MYLK1 gene. This gene encodes telokin, non-muscle and the smooth muscle isoforms of MLCK. MLCK gene is mapped to the human chromosome location 3q21.1.

In immunoblotting, the antibody recognizes the myosin light chain kinase of smooth muscle from various sources including chicken gizzard, turkey gizzard, and pig stomach as well as the myosin light chain kinase of non-muscle cells such as cultured fibroblasts.

chicken gizzard myosin light chain kinase.

Monoclonal anti-myosin light chain kinase antibody has been used in:

• immunohistochemistry
• immunoblot (diluted 1:10,000) analysis
• flow cytometry

Myosin Light Chain Kinase (MLCK) has a pivotal role in phosphorylating myosin regulatory light chains and hence facilitates its interaction with actin filaments for smooth muscle contraction. Monoclonal anti-myosin light chain kinase antibody can be used for studying the function of the enzyme and its interactions with other cell components. It can also be used in microarray and immunoprecipitation. Mouse anti-myosin light chain kinase antibody reacts specifically with myosin light chain kinase present in smooth muscle of chicken gizzard, turkey gizzard, and pig stomach. This product also shows reactivity for myosin light chain kinase of non-muscle cells like cultured fibroblasts.

The catalytic subunit of cardiac and skeletal muscle myosin light chain kinase is in the range of 80-95 kDa. Proteolysis of the myosin light chain kinase from chicken gizzard produces a 64 kDa fragment that neither binds to Ca²⁺/calmodulin nor exhibits catalytic activity and a 61 kDa peptide that is active in the absence of Ca²⁺/calmodulin.

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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