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M7802

Sigma-Aldrich

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-ERK, Anti-ERK-2, Anti-ERK2, Anti-ERT1, Anti-MAPK2, Anti-NS13, Anti-P42MAPK, Anti-PRKM1, Anti-PRKM2, Anti-p38, Anti-p40, Anti-p41, Anti-p41mapk, Anti-p42-MAPK

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About This Item

MDL-Nummer:
MFCD01633612
UNSPSC-Code:
12352203
NACRES:
NA.44

Biologische Quelle

mouse

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

ERK-PT115, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa

Speziesreaktivität

rat, human

Konzentration

~2 mg/mL

Methode(n)

capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol

Isotyp

IgG1

UniProt-Hinterlegungsnummer

P28482

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... MAPK1(5594) , MAPK3(5595)
rat ... Mapk1(116590) , Mapk3(50689)

Allgemeine Beschreibung

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

Spezifität

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr183 in ERK-2).

Immunogen

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Anwendung

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:
  • immunoblotting
  • western blotting
  • immunofluorescence
  • enzyme linked immunosorbent assay (ELISA)
  • immunochemistry

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Evaluation of the role of MAPK1 and CREB1 polymorphisms on treatment resistance, response and remission in mood disorder patients
Calati R, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 44, 271-278 (2013)
Leader cell positioning drives wound-directed collective migration in TGFbeta-stimulated epithelial sheets
Chapnick DA and Liu X
Molecular Biology of the Cell, 25(10), 1586-1593 (2014)
Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis
Huang YWA, et al.
Cell, 148(5), 933-946 (2012)
Transcriptional consequences of 16p11. 2 deletion and duplication in mouse cortex and multiplex autism families
Blumenthal I, et al.
American Journal of Human Genetics, 94(6), 870-883 (2014)
MAPKs: new JNK expands the group
Davis RJ
Trends in Biochemical Sciences, 19, 1027-1037 (1994)
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