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M6697

Sigma-Aldrich

Anti-MLKL (58-70) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(e):

Anti-mixed lineage kinase domain-like (Homo sapiens)

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~54 kDa

Speziesreaktivität

human

Methode(n)

western blot: 1:250-1:500

UniProt-Hinterlegungsnummer

Q8NB16

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... MLKL(197259)

Allgemeine Beschreibung

MLKL (mixed lineage kinase domain-like) is a protein that belongs to the protein kinase superfamily. It facilitates necrosis signaling downstream of the kinase RIP3 (receptor-interacting serine-threonine kinase 3) . Anti-MLKL (58-70) antibody can be used in western blotting. Rabbit anti- MLKL (58-70) antibody reacts specifically with MLKL protein.
MLKL is encoded by the gene mapped to human chromosome 16q23. Activated MLKL is localized on the cell membrane.

Immunogen

synthetic peptide corresponding to amino acids 58-70 of human MLKL.

Anwendung

Anti-MLKL (58-70) antibody produced in rabbit has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
western blot: 1:250-1:500

Biochem./physiol. Wirkung

Mixed lineage kinase domain-like protein (MLKL) primarily causes receptor-interacting protein (RIP) kinase-dependent necroptosis. However, during hepatitis, it results in programmed hepatocellular necrosis, which is independent of RIPK3. MLKL also participates in endosomal trafficking and in the formation of extracellular vesicles.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Sara R Oliveira et al.
Cell death discovery, 4, 10-10 (2018-08-01)
Necroptosis is a regulated form of necrosis, which may be critical in the pathogenesis of neurodegenerative diseases. Neuroinflammation, characterized by the activation of glial cells such as microglia, is closely linked with neurodegenerative pathways and constitutes a major mechanism of
Characterization of RIPK3-mediated phosphorylation of the activation loop of MLKL during necroptosis.
Rodriguez DA, et al.
Cell Death and Differentiation, 23(1), 76-76 (2016)
Tommaso Selmi et al.
BMC cancer, 15, 357-357 (2015-05-06)
ZFP36 is an mRNA binding protein that exerts anti-tumor activity in glioblastoma by triggering cell death, associated to an increase in the stability of the kinase RIP1. We used cell death assays, size exclusion chromatography, Co-Immunoprecipitation, shRNA lentivectors and glioma
ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly.
Selmi T, et al.
BMC Cancer, 15(1), 357-357 (2015)
MLKL activation triggers NLRP3-mediated processing and release of IL-β independently of gasdermin-D
Gutierrez KD, et al.
Journal of Immunology, 47(1), 1601757-1601757 (2017)
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