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L3023

Sigma-Aldrich

Lipopolysaccharide aus Escherichia coli O111:B4

Detoxified

Synonym(e):

Endotoxin, LPS

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About This Item

EG-Nummer:
297-473-0
MDL-Nummer:
MFCD00164401
UNSPSC-Code:
12352201
NACRES:
NA.25

Biologische Quelle

Escherichia coli (O111:B4)

Qualitätsniveau

200

Form

lyophilized powder

Farbe

white to beige

Löslichkeit

water: soluble

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

This product is extracted from E. coli serotype O111:B4 and purified by chromatography. The source strain is from a private collection. The lipid A moiety has been partially delipidated by alkaline hydrolysis. Removal of the fatty acid portions of lipid A results in a detoxified LPS with an endotoxin level about 10,000 times lower than that of the parent LPS.

Anwendung

Lipopolysaccharide (LPS) sind charakteristische Bestandteile der Zellwand von gramnegativen Bakterien. LPS und seine Lipid-A-Gruppe stimulieren Zellen des angeborenen Immunsystems durch den Toll-like-Rezeptor 4 (TLR4). TLR4 gehört zur Toll-like-Rezeptor-Proteinfamilie, die häufige Pathogen-assoziierte molekulare Strukturen (PAMPs) erkennt.

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

Chromatographically purified; delipidized by alkaline hydrolysis
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Lipopolysaccharide aus Escherichia coli O111:B4, Detoxified

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Lipopolysaccharide aus Escherichia coli O111:B4, Ready Made solution, 1 mg/mL

Piktogramme

Skull and crossbones
GHS06

Signalwort

Danger

H-Sätze

H300

Gefahreneinstufungen

Acute Tox. 2 Oral

Lagerklassenschlüssel

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Hua Qiu et al.
Drug design, development and therapy, 13, 589-600 (2019-02-28)
Jie-Du-Hua-Yu (JDHY) granule is a combination of six traditional Chinese medicines with known therapeutic effect in treating acute liver failure (ALF). The aim of this study was to investigate the amelioration efficacy of JDHY in lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced ALF in rat
Carol L Ladner-Keay et al.
Prion, 10(6), 466-483 (2016-12-03)
Conversion of native cellular prion protein (PrPc) from an α-helical structure to a toxic and infectious β-sheet structure (PrPSc) is a critical step in the development of prion disease. There are some indications that the formation of PrPSc is preceded
Gabrielle R Andrade et al.
Human vaccines & immunotherapeutics, 10(10), 2864-2874 (2014-12-09)
E. coli O111 strains are responsible for outbreaks of blood diarrhea and hemolytic uremic syndrome throughout the world. Because of their phenotypic variability, the development of a vaccine against these strains which targets an antigen that is common to all
Rhonda H Wilson et al.
American journal of respiratory and critical care medicine, 180(8), 720-730 (2009-08-08)
In humans, immune responses to inhaled aeroallergens develop in the lung and draining lymph nodes. Many animal models of asthma bypass this route and instead use intraperitoneal injections of allergen using aluminum hydroxide as an adjuvant. We investigated whether allergic
Mohamed El Gazzar et al.
Molecular and cellular biology, 29(7), 1959-1971 (2009-01-23)
Epigenetic silencing of tumor necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) transcription occurs in blood leukocytes of animals and humans after the initiation of severe systemic inflammation (SSI). We previously reported that the epigenetic signature requires induction of NF-kappaB
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Verwandter Inhalt

Lipopolysaccharides

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.

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