Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

H9661

Sigma-Aldrich

Hepatocyte Growth Factor human

HGF, recombinant, expressed in NSO cells, suitable for cell culture

Synonym(e):

HGF, Hepatopoietin A, Scatter Factor

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

MDL-Nummer:
MFCD00217422
UNSPSC-Code:
12352202
NACRES:
NA.77

Biologische Quelle

human

Qualitätsniveau

200

Rekombinant

expressed in NSO cells

Assay

≥95% (SDS-PAGE)

Form

lyophilized powder

Wirksamkeit

20-40 ng/mL

Qualität

endotoxin tested

Mol-Gew.

70-80 kDa by SDS-PAGE (non-reducing)

Verpackung

pkg of 5 and 25 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

<1.0 EU/μg

UniProt-Hinterlegungsnummer

P14210

Lagertemp.

−20°C

Angaben zum Gen

human ... HGF(3082)

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Verwandte Kategorien

Allgemeine Beschreibung

Recombinant, human hepatocyte growth factor (HGF) is produced from the 728 amino acid residue variant of the human pre-pro-HGF protein 6 and expressed in a mouse myeloma cell line NSO.The HGF gene is mapped to human chromosome 7q21.1. The encoded protein is a mesenchymal-fibroblast derived cytokine.

Anwendung

Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.

Biochem./physiol. Wirkung

HGF′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth. Abnormal and defective HGF causes preeclampsia due to placental insufficiency. HGF exhibits its multifunctions through c-MET (proto-oncogene tyrosine kinase receptor). HGF is known to induce human umbilical vein endothelial cell proliferation and vasculogenic mimicry migration in gastric cancer cells, thus serving as a good target for cancer therapy. Upregulation of the HGF gene is observed in advanced stages of cancer, such as pancreatic adenocarcinoma.
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Sonstige Hinweise

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.

Hinweis zur Analyse

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kunden haben sich ebenfalls angesehen

Slide 1 of 5

1 of 5

Transforming Growth Factor-&#946;1 human TGF-&#946;1, recombinant, expressed in CHO cells, powder, suitable for cell culture

Sigma-Aldrich

T7039

Transforming Growth Factor-β1 human

hEGF EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

E9644

hEGF

Nicotinamid BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

N0636

Nicotinamid

Nicotinamid &#8805;99.5% (HPLC)

Sigma-Aldrich

72340

Nicotinamid

Dexamethason powder, BioReagent, suitable for cell culture, &#8805;97%

Sigma-Aldrich

D4902

Dexamethason

Correlation between HGF/c-Met and Notch1 signaling pathways in human gastric cancer cells
Huang KH, et al.
Oncology Reports, 40(1), 294-302 (2018)
Dual inhibition of Hedgehog and c-Met pathways for pancreatic cancer treatment
Rucki AA, et al.
Molecular Cancer Therapeutics (2017)
Negative control of the HGF/c-MET pathway by TGF-beta: a new look at the regulation of stemness in glioblastoma
Papa E, et al.
Cell Death & Disease, 8(12), 3210-3210 (2017)
HGF derived from cancer-associated fibroblasts promotes vascularization in gastric cancer via PI3K/AKT and ERK1/2 signaling
Ding X, et al.
Oncology Reports (2018)
Prognostic implication of MET overexpression in myxofibrosarcomas: an integrative array comparative genomic hybridization, real-time quantitative PCR, immunoblotting, and immunohistochemical analysis
Lee JC, et al.
Modern Pathology, 23(10), 1379-1379 (2010)
Alle zugehörigen Dokumente anzeigen

Artikel

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.