GE28-9537-66
HiTrap® TALON® Crude
Cytiva 28-9537-66, pack of 5 × 1 mL
About This Item
Empfohlene Produkte
ligand
TALON® Superflow Cobalt-IMAC
Haltbarkeit
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Verpackung
pack of 5 × 1 mL
Hersteller/Markenname
Cytiva 28-9537-66
Parameter
1 ml/min - 4 ml/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
3 bar
Säule ID
7 mm
Matrix
6% cross-linked agarose
Partikelgröße
60-160 μm
cleaning
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
Kapazität
≥20 mg binding capacity (histidine-tagged protein)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
Lagertemp.
2-8°C
Verwandte Kategorien
Allgemeine Beschreibung
Anwendung
HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.
Leistungsmerkmale und Vorteile
- Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of Proteins.
- HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning-Warning
H-Sätze
Gefahreneinstufungen
Flam. Liq. 3
Lagerklassenschlüssel
3 - Flammable liquids
WGK
WGK 1
Flammpunkt (°F)
100.4 - 109.4 °F
Flammpunkt (°C)
38 - 43 °C
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Artikel
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Protokolle
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
How to isolate proteins from inclusion bodies using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.