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GE17-5478-51

HiTrap® Protein L

Cytiva 17-5478-51, pack of 5 × 1 mL

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

recombinant protein L (E. coli), mammalian free

Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Verpackung

pack of 5 × 1 mL

Hersteller/Markenname

Cytiva 17-5478-51

Lagerbedingungen

(20% Ehtanol)

Parameter

5 bar

Säule ID

7 mm

Matrix

rigid, highly cross-linked agarose

Durchschnittliche Teilgröße

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

working range

2-10(pH interval where the medium can be operated without significant change in function.)

Eignung

suitable for bioprocess medium

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

HiTrap® Protein L columns are packed with Capto™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments.

Anwendung

HiTrap® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs).

Leistungsmerkmale und Vorteile

  • Captures a wide range of antibody fragments
  • Allows a platform approach for the purification of antibody fragment
  • Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments
  • Reduces process time and amount of resin
  • Suitable for process development and small-scale purification
  • HiTrap® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA® system.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

Performing a Purification of Antibody Fragments with Capto™ L Products

This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.

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