GE17-5281-01
GSTrap™ High Performance
Cytiva 17-5281-01, pack of 5 × 1 mL
About This Item
Empfohlene Produkte
ligand
glutathione
Haltbarkeit
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Verpackung
pack of 5 × 1 mL
Hersteller/Markenname
Cytiva 17-5281-01
Parameter
<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi (H2O at room temperature.)
Bettgröße
7 mm × 25 mm
Bettvolumen
1 mL
Säule ID
7 mm
Matrix
highly cross-linked 6% agarose
Durchschnittliche Teilgröße
34 μm
cleaning
3-12
working range
3-12
Kapazität
>7 mg/mL binding capacity(H2O at room temperature.)
Verwandte Kategorien
Allgemeine Beschreibung
Anwendung
GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.
Leistungsmerkmale und Vorteile
- High resolution purification of GST-tagged Proteins.
- Mild elution conditions preserving Protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Protokolle
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
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