GE17-5195-01
HiTrap® Phenyl High Performance
Cytiva 17-5195-01, pack of 5 × 5 mL
About This Item
Empfohlene Produkte
ligand
phenyl
Haltbarkeit
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Verpackung
pack of 5 × 5 mL
Hersteller/Markenname
Cytiva 17-5195-01
Parameter
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
Bettgröße
16 mm × 25 mm
Bettvolumen
5 mL
Säule ID
16 mm
Matrix
6% cross-linked agarose
Partikelgröße
24-44 μm
Durchschnittliche Teilgröße
34 μm
cleaning
2-14
working range
3-13
Eignung
suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium
Verwandte Kategorien
Allgemeine Beschreibung
Leistungsmerkmale und Vorteile
- Provide convenient and fast small-scale protein purifications.
- Phenyl Sepharose™ High Performance is a well-established aromatic HIC medium, designed for intermediate and polishing steps where high resolution has priority.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as AKTA design.
- High flow rates and high capacities.
- Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
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Artikel
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.
This page discusses column packing and preparation techniques for reverse phase chromatography.
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Protokolle
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page discusses various aspects of sample preparation for chromatographic purification.
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