Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

GE17371201

Ni Sepharose™ Excel

Cytiva 17371201, 90 μm avg. part. size

Synonym(e):

Ni Sepharose™ Excel

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

Hersteller/Markenname

Cytiva 17371201

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

90 μm

cleaning in place

2-14

working range

2-12

Kapazität

≥10 mg binding capacity (histidine-tagged protein)()Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6-tagged pure protein (Mr 43 000) in EX-CELL 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)

Verwandte Kategorien

Allgemeine Beschreibung

Ni Sepharose™ excel is an immobilized metal ion affinity chromatography (IMAC) resin precharged with nickel ions very strongly bound to a chelating ligand. This histidine-tagged (his-tagged) protein purification medium ensures minimized Ni-leakage and maximized protein recovery when samples contain stripping agents. Samples that usually cause stripping of metal ions can therefore be loaded to the medium. The nickel ions have been shown to remain bound to the chelating ligand even after incubation for 24 hours in 10 mM EDTA. Ni Sepharose excel is designed primarily for capture and purification of his-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells by IMAC.

Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures. The flow properties of the medium make it excellent for purifications in a wide range of scales and allow loading of large sample volumes, enabling purification of low concentrations of target proteins at large volumes.

Leistungsmerkmale und Vorteile

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Choose between several formats for screening and preparative purification of histidine-tagged proteins

Lagerung und Haltbarkeit

Store at 4 to 30 °C (20% ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Environment
GHS09

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kunden haben sich ebenfalls angesehen

Slide 1 of 10

1 of 10

HIS-Select® HF-Nickel-Affinitätsgel

Millipore

H0537

HIS-Select® HF-Nickel-Affinitätsgel

Ni Sepharose™ 6 Fast Flow Cytiva 17-5318-02, pack of 100 mL

GE17-5318-02

Ni Sepharose™ 6 Fast Flow

Ni Sepharose™ 6 Fast Flow Cytiva 17-5318-06, pack of 5 mL

GE17-5318-06

Ni Sepharose™ 6 Fast Flow

cOmplete™ His-Tag-Aufreinigungsharz suspension, suitable for protein purification, matrix Sepharose-CL 6B

Roche

COHISR-RO

cOmplete™ His-Tag-Aufreinigungsharz

Capto™ Q Cytiva 17-5316-02, pack of 100 mL

GE17-5316-02

Capto™ Q

IMAC Sepharose™ 6 Fast Flow Cytiva 17-0921-07, pack of 25 mL

GE17-0921-07

IMAC Sepharose™ 6 Fast Flow

Millipore

Millipore

70666

Ni-NTA His•Bind® Resin

His SpinTrap™ Cytiva 28-4013-53

GE28-4013-53

His SpinTrap™

cOmplete™-His-Tag-Aufreinigungssäule Prepacked, ready-to-use chromatography column

Roche

COHISC-RO

cOmplete™-His-Tag-Aufreinigungssäule

HIS-Select® Nickel-Affinitätsgel (1:1 suspension in a 20% ethanol solution)

Millipore

P6611

HIS-Select® Nickel-Affinitätsgel

Artikel

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Protokolle

Tag Removal by Enzymatic Cleavage

This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.