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F0926

Sigma-Aldrich

Fetales Kälberserum

USDA approved, sterile-filtered, suitable for cell culture

Synonym(e):

FBS, FCS, Seren, Serum

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About This Item

MDL-Nummer:
MFCD00132239
UNSPSC-Code:
12352207
NACRES:
NA.71

Biologische Quelle

bovine fetus

Qualitätsniveau

500

Sterilität

sterile-filtered

Produktlinie

FBS Classic

Qualität

USDA approved

Zusammensetzung

Hemoglobin, ≤25 mg/dL

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

≤10 EU/mL endotoxin

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. It is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells.

Anwendung

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Fetal Bovine Serum has been used for the following cell cultures:
  • Primary Culture Human Macrophages (mononuclear cells were re-suspended in Roswell Park Memorial Institute (RPMI) medium containing fetal bovine serum (FBS) along with other components).
  • Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components).
  • Isolation and 3D culture of primary mammary epithelial organoids
  • Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors)

Eignung

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Hinweis zur Analyse

  • Endotoxin- und Hämoglobin-getestet
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Sonstige Hinweise

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

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