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Merck

E0760

Sigma-Aldrich

Endothelial cell growth supplement from bovine pituitary

ECGS, suitable for cell culture, BioReagent

Synonym(e):

ECGS, Endothelial Mitogen

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.77

Biologische Quelle

bovine pituitary glands

Qualitätsniveau

Produktlinie

BioReagent

Form

powder

Wirksamkeit

2-300 μg/mL ED50/EC50

Qualität

endotoxin tested

Verpackung

pkg of 15 mg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

≤20 EU/vial (LAL test)

Farbe

brown to tan

Löslichkeit

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Anwendung

Endothelial cell growth supplement from bovine pituitary has been used:
  • as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
  • as a supplement in medium 199 for endothelial cell culture
  • as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
  • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem./physiol. Wirkung

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Piktogramme

Health hazard

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Repr. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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