Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

D8940

Sigma-Aldrich

Anti-Destrin/ADF (AE-14) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti-Actin Depolymerizing Factor

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

MDL-Nummer:
MFCD03791499
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

mouse, rat, human

Konzentration

~0.8-1.2 mg/mL

Methode(n)

microarray: suitable
western blot: 1:600 using whole extracts of human A431 epidermoid carcinoma cells, rat PC-12 pheochromocytoma cells, or mouse NIH3T3 fibroblasts

UniProt-Hinterlegungsnummer

P60981

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... DSTN(11034)
mouse ... Dstn(56431)
rat ... LOC296197(296197)

Allgemeine Beschreibung

Destrin/ADF and cofilin are widely distributed in tissues of eukaryotes and both contain a nuclear localization sequence. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. This gene is located on human chromosome 20p12.
Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘Hirano bodies′ in certain brain neurons of dementia patients.

Immunogen

synthetic peptide corresponding to amino acids 137-150 of human destrin/ADF.

Anwendung

Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:600 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and mouse NIH/3T3 fibroblasts, and a chemiluminescent detection reagent.

Biochem./physiol. Wirkung

Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca2+ independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Produkt-Auswahlhilfe.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Genome-wide association study of antiphospholipid antibodies
Kamboh MI, et al.
Autoimmune diseases, 2013 (2013)
The ADF/cofilin family: actin-remodeling proteins
Maciver SK and Hussey PJ
Genome Biology, 3(5), reviews3007-reviews3001 (2002)
E Nishida et al.
Biochemistry, 24(23), 6624-6630 (1985-11-05)
An Mr 19 000 protein (destrin) that has the ability to rapidly depolymerize F-actin in a stoichiometric manner was purified from porcine kidney by sequential chromatography on DNase I-agarose, hydroxyapatite, and Sephadex G-75. Its actin-depolymerizing activity is reversibly controlled by
K Moriyama et al.
The Journal of biological chemistry, 265(10), 5768-5773 (1990-04-05)
Destrin is a mammalian 19-kDa protein that rapidly depolymerizes F-actin in a stoichiometric manner. In this study, we isolated cDNA clones coding for destrin from a porcine brain cDNA library. The deduced amino acid sequence of destrin is 165 residues
M E Adams et al.
Biochemistry, 29(32), 7414-7420 (1990-08-14)
Chick brain actin depolymerizing factor (ADF) is a 19-kDa protein that severs actin filaments and binds actin monomers. We have obtained a cDNA encoding ADF by screening a chick embryo lambda gt11 cDNA library with both a rabbit anti-ADF antiserum
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.