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D2906

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym(e):

DME/F-12, 1:1 Mischung

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About This Item

MDL-Nummer:
MFCD00217417
UNSPSC-Code:
12352207
NACRES:
NA.75

product name

Dulbecco Modifiziertes Eagle-Medium/Nährstoffmischung F-12 Schinken, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Qualitätsniveau

500

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Anwendung

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • Hep-2 cells
  • mouse fibroblast L‐cells
  • L‐ Wnt family member 3A (Wnt3a) cells
  • primary buffalo embryonic fibroblast (BEF)

Menge

Formulated to contain 15.6 grams of powder per liter of medium.

Rekonstituierung

Supplement with 1.2 g/L sodium bicarbonate.

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WGK

WGK 1

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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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