C0888
94070403, from human blood
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About This Item
UNSPSC-Code:
41106514
Empfohlene Produkte
Biologische Quelle
human blood
Nummer des HRC-Gremiums
1
Ethnizität
UK Caucasian
Geschlecht
male
Wachstumsmodus
Suspension
Karyotyp
Not Specified
Morphologie
Lymphoblastoid
Methode(n)
cell culture | mammalian: suitable
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Ursprung der Zelllinie
UK Caucasian Male
Beschreibung der Zelllinie
A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
Anwendung
As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.
Nährmedium
RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subkultur-Routine
Maintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.
Sonstige Hinweise
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