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PC-9 (formerly known as PC-14)

90071810

Synonym(e):

PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells

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About This Item

UNSPSC-Code:
41106514

Biologische Quelle

human lung

Wachstumsmodus

Adherent/suspension

Karyotyp

Not specified

Morphologie

Heterogeneous mixture of round cells and spindle shaped cells

Produkte

Not specified

Rezeptoren

Not specified

Methode(n)

cell culture | mammalian: suitable

Relevante Krankheit(en)

cancer

Versandbedingung

dry ice

Lagertemp.

−196°C

Verwandte Kategorien

Ursprung der Zelllinie

Human adenocarcinoma

Beschreibung der Zelllinie

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

Anwendung

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.
Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Seed cells at 2-4 x 10,000 cells/cm2; 5% CO2; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

Sonstige Hinweise

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Artikel

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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