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Sigma-Aldrich

Photobiotin -acetat (Salz)

For labeling DNA probes for hybridizations

Synonym(e):

Biotin-{3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]-propylamid} -acetat (Salz), N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propandiamin -acetat (Salz)

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About This Item

Empirische Formel (Hill-System):
C23H35N9O4S · C2H4O2
CAS-Nummer:
96087-38-6
Molekulargewicht:
593.70
MDL-Nummer:
MFCD00210085
UNSPSC-Code:
12352200
PubChem Substanz-ID:
24890604
NACRES:
NA.52

Qualität

for molecular biology

Qualitätsniveau

200

Assay

≥95%

Form

film or powder

Löslichkeit

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

Lagertemp.

−20°C

SMILES String

CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]

InChI

1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1

InChIKey

FFBLNTOMOSLSQM-AYEYRVMASA-N

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Allgemeine Beschreibung

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Anwendung

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Biochem./physiol. Wirkung

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.
Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Vorsicht

Light sensitive.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Kwang Kyu Kim et al.
International journal of systematic and evolutionary microbiology, 55(Pt 5), 2075-2079 (2005-09-17)
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H Zreiqat et al.
Molecular biotechnology, 10(2), 107-113 (1998-11-20)
The universal quantitation of the DNA hybridization reaction has been a goal sought by many researchers. Part of this search has been the need to develop a rapid, sensitive, easy-to-perform, and quantitative method to measure the abundance of specific mRNAs
Kwang Kyu Kim et al.
International journal of systematic and evolutionary microbiology, 58(Pt 8), 1916-1921 (2008-08-05)
Two 4-chlorophenol-degrading bacteria, strains 4C1-aT and 4C1-b, were isolated from sewage flowing into Geumho River near the Daegu industrial complex in Korea. Cells of the strains were Gram-positive and non-motile, displayed a rod-coccus life cycle and formed creamy white colonies
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