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Sigma-Aldrich

Hoechst 33258 -Lösung

1 mg/mL in H2O, ≥98.0% (HPLC)

Synonym(e):

Bisbenzimid H 33258 -Lösung

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About This Item

Empirische Formel (Hill-System):
C25H24N6O · 3HCl
CAS-Nummer:
23491-45-4
Molekulargewicht:
533.88
UNSPSC-Code:
12171500
PubChem Substanz-ID:
329770334
NACRES:
NA.32

Qualitätsniveau

100

Assay

≥98.0% (HPLC)

Form

liquid

Konzentration

1 mg/mL in H2O

Löslichkeit

DMF: soluble
H2O: soluble

Fluoreszenz

λex 355 nm; λem 465 nm in TE buffer; DNA

Lagertemp.

2-8°C

SMILES String

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChIKey

SMNPLAKEGAEPJD-UHFFFAOYSA-N

Allgemeine Beschreibung

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

Anwendung

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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