68221
Abberior® STAR 440SXP, NHS-Ester
for long Stokes STED and 2-color STED application
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352108
NACRES:
NA.32
Empfohlene Produkte
Qualitätsniveau
Assay
≥80.0% (degree of coupling)
Form
solid
Mol-Gew.
Mw 597.5 g/mol
Löslichkeit
DMF: 1 mg/mL, clear
Fluoreszenz
λex 440 nm; λem 511 nm±10 nm in PBS, pH 7.4
Lagertemp.
−20°C
Allgemeine Beschreibung
Absorption Maximum, λmax: 433 nm (MeOH)
432 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 36,000 M-1cm-1 (MeOH)
33,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 502 nm (MeOH),
511 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 − 620 nm
Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)
432 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 36,000 M-1cm-1 (MeOH)
33,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 502 nm (MeOH),
511 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 − 620 nm
Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)
Anwendung
- Abberior™ STAR 440SXP goat anti-mouse antibody has been used for STED (stimulated emission depletion) microscopy imaging of primary hippocampal cells.
- Secondary anti-mouse antibody conjugated with Abberior™ STAR 440SXP has been used for gated STED microscopy imaging of viral capsid.
- Abberior™ STAR 440SXP goat anti-rabbit antibody has been used for two-color STED microscopy imaging of olfactory epithelium.
Eignung
Designed and tested for fluorescent super-resolution microscopy
Sonstige Hinweise
Rechtliche Hinweise
6538 is a trademark of American Type Culture Collection
abberior is a registered trademark of Abberior GmbH
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Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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S W Hell et al.
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We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
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Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
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