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62288

Sigma-Aldrich

Lipase B Candida antarctica, rekombinant aus Aspergillus oryzae

powder, beige, ~9 U/mg

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About This Item

CAS-Nummer:
9001-62-1
EC-Nummer:
3.1.1.3 (BRENDA, IUBMB)
EG-Nummer:
232-619-9
MDL-Nummer:
MFCD00131509
UNSPSC-Code:
12352204
NACRES:
NA.54

Rekombinant

expressed in Aspergillus oryzae

Qualitätsniveau

100

Form

powder

Spezifische Aktivität

~9 U/mg

Mol-Gew.

33 kDa

Farbe

beige

Lagertemp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChIKey

QWZUIMCIEOCSJF-CHHCPSLASA-N

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Allgemeine Beschreibung

Candida antarctica lipase B (CalB) is structurally similar to several other lipases and has a flexible lid. It is made up of 317 amino acids and has a molecular weight of 33 kDa. Lipase B is a member of the alpha/beta hydrolase-fold family.

Anwendung

Lipase B Candida antarctica, recombinant from Aspergillus oryzae has been used:
  • as a standard to characterize the enzymatic properties of D5-CalB
  • as an efficient biocatalyst to start the reaction to obtain (R)-ester via esterification of racemic secondary alcohol
  • to investigate a “green” recycling route for polybutylene succinate (PBS) based on reactive extrusion
  • to compare the esterification yield with adsorbed CaLB (aCaLB) and covalently immobilized CaLB (cCaLB)

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem./physiol. Wirkung

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions.
Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol Buttersäure pro Minute bei pH 8.0 und 40°C freisetzt (Tributyrin, Fluka®-Nr. 91010, als Substrat)

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli
Kim S K, et al.
Biotechnology and Bioengineering, 112(2), 346-355 (2015)
Sun-Ki Kim et al.
Biotechnology and bioengineering, 112(2), 346-355 (2014-09-04)
Escherichia coli is the best-established microbial host strain for production of proteins and chemicals, but has a weakness for not secreting high amounts of active heterologous proteins to the extracellular culture medium, of which origins belong to whether prokaryotes or
Structural behavior of Candida antarctica lipase B in water and supercritical carbon dioxide: A molecular dynamic simulation study
Housaindokht M R, et al.
Journal of Supercritical Fluids, 63, 180-186 (2012)
A green method for polybutylene succinate recycling: Depolymerization catalyzed by lipase B from Candida antarctica during reactive extrusion
Jbilou F, et al.
European Polymer Journal, 68, 207-215 (2015)
A novel self-activation mechanism of Candida antarctica lipase B
Luan B and Zhou R
Physical Chemistry Chemical Physics, 19(24), 15709-15714 (2017)
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