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B0126

Sigma-Aldrich

Bromphenolblau

titration: suitable

Synonym(e):

3′,3″,5′,5″-Tetrabrom-phenolsulfonphthalein, BPB, Bromphenolblau, Sulton Form

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About This Item

Empirische Formel (Hill-System):
C19H10Br4O5S
CAS-Nummer:
115-39-9
Molekulargewicht:
669.96
Beilstein:
61698
EG-Nummer:
204-086-2
MDL-Nummer:
MFCD00005875
UNSPSC-Code:
12171500
PubChem Substanz-ID:
24891504
NACRES:
NA.47

Agentur

suitable for SM 2310
suitable for SM 2320

Qualitätsniveau

200

Form

powder

Methode(n)

titration: suitable

Farbe

faint beige to very dark beige
faint orange to very dark orange

visual transition interval

3.0-4.6, yellow-green to blue

mp (Schmelzpunkt)

273 °C

Löslichkeit

methanol: 10 mg/mL

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

room temp

SMILES String

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChIKey

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Allgemeine Beschreibung

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Anwendung

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number

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