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60983

Millipore

Kovac-Reagenz für Indole

suitable for microbiology

Synonym(e):

4-Dimethylamino-benzaldehyd -Lösung, Indol-Reagens nach Kovac

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About This Item

Empirische Formel (Hill-System):
C9H11NO
CAS-Nummer:
100-10-7
Molekulargewicht:
149.19
Beilstein:
4132845
MDL-Nummer:
MFCD00003381
UNSPSC-Code:
41171621
PubChem Substanz-ID:
329759335
NACRES:
NA.85

Haltbarkeit

limited shelf life, expiry date on the label

Qualitätsniveau

100

Zusammensetzung

n-butanol
4-dimethylaminobenzaldehyde
hydrochloric acid

Methode(n)

microbe id | specific enzyme detection: suitable

Brechungsindex

n20/D 1.400-1.420

Anwendung(en)

agriculture
clinical testing
environmental
food and beverages
microbiology

Lagertemp.

2-8°C

Eignung

Escherichia coli
coliforms

SMILES String

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChIKey

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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Allgemeine Beschreibung

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Anwendung

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Biochem./physiol. Wirkung

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Central nervous system, Respiratory system

WGK

WGK 1

Flammpunkt (°F)

96.8 °F

Flammpunkt (°C)

36 °C

Persönliche Schutzausrüstung

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

EU REACH Annex XVII (Restriction List)

CAS No.

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Stavros G Manios et al.
Meat science, 101, 25-32 (2014-12-03)
The effect of common handling practices (i.e., freezing, thawing and cooking) of beef patties on the survival of Salmonella spp. and Escherichia coli O157:H7, was evaluated. Inoculated ground beef was stored at -22 °C for 5 and 75 days. After
Ragnhildur Gunnarsdóttir et al.
Environmental science & technology, 46(22), 12408-12416 (2012-11-02)
Wastewater treatment in many Arctic regions is inadequate, even nonexisting. Natural freezing of wastewater in those areas may be beneficial for reduction of microorganisms. The aim of this study was to investigate the effect of long-term freezing, and repeated freezing
Jun-Min Guo et al.
Journal of agricultural and food chemistry, 58(11), 6556-6561 (2010-05-15)
A simple colorimetric method for the differentiation of indoleacetic acid (IAA) and indolebutyric acid (IBA) in plant samples is described. The color change is based upon the reaction between the auxins and p-(dimethylamino)benzaldehyde (PDAB, Ehrlich reagent) following the electrophilic substitution
G P Kushto et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 54A(6), 799-819 (1998-07-17)
Results of a normal coordinate analysis based on infrared and Raman spectra of six isotopic forms of 4-(dimethylamino)benzaldehyde are reported (DABA, DABA-CDO, DABA-13CDO, DABA-13CHO, DABA-CHO18O, and DABA-3,5-D). Cs point group symmetry has been applied and all motions except the internal
N T Yatsimirskaya et al.
Analytical biochemistry, 229(2), 249-255 (1995-08-10)
Yields of colored Schiff base products of the reversible condensation of 6-aminopenicillanic and 7-aminocephalosporanic acids with para-dimethylaminobenzaldehyde in a weakly acid aqueous solution strongly increase in the presence of micelles of sodium dodecyl sulfate. The binding constants of reactants and
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