10109886001
Roche
Trypsin Inhibitor
from soybean
Synonym(e):
trypsin
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About This Item
UNSPSC-Code:
12352207
Empfohlene Produkte
Biologische Quelle
soybean
Qualitätsniveau
Form
lyophilized
Mol-Gew.
Mr 20,100
Verpackung
pkg of 50 mg
Hersteller/Markenname
Roche
Konzentration
1 mg/mL
Methode(n)
cell culture | mammalian: suitable
pH-Wert
3.8-4.5
Löslichkeit
water: soluble
Lagertemp.
2-8°C
Verwandte Kategorien
Allgemeine Beschreibung
Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.
Spezifität
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).
Anwendung
Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- in the buffer for hepatocyte isolation
- to inhibit trypsin in Corneal epithelial cells
- to inhibit papain activity while treating hippocampal neural stem cells (NSCs)
Biochem./physiol. Wirkung
Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.
Spezifikationen
Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)
Isoelectric point: 4.5
Isoelectric point: 4.5
Einheitendefinition
1 U = 270 S & T-units or 90 USP-units
Angaben zur Herstellung
Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Rekonstituierung
Sensitive to heat, alkaline pH, and protein-precipitating compounds.
Lagerung und Haltbarkeit
Store at 2 to 8 °C. (Store dry!)
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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