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10102105001

Roche

Adenosine Deaminase (ADA)

from calf intestine

Synonym(e):

ADA

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About This Item

EC-Nummer:
3.5.4.4 (BRENDA, IUBMB)
UNSPSC-Code:
12352204

Biologische Quelle

bovine (calf) intestine

Qualitätsniveau

100

Form

solution

Spezifische Aktivität

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

Mol-Gew.

230-440 kDa (high)
 30-47 kDa (low )

Verpackung

pkg of 2 mL (10 mg)

Hersteller/Markenname

Roche

Optimaler pH-Wert

7.0-7.4

Versandbedingung

wet ice

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Anwendung

Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Adenosine deaminase has been used to pre-treat primary Kupffer cells.

Biochem./physiol. Wirkung

Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Qualität

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physikalische Form

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6

Angaben zur Herstellung

Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

WGK

WGK 1

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Adenosin suitable for cell culture, BioReagent

Sigma-Aldrich

A4036

Adenosin

Pdx-1 is required for activation in vivo from a duodenum-specific enhancer
Dusing MR, et al.
The Journal of biological chemistry, 276(17), 14434-14442 (2001)
Adenosine deaminase deficiency: a review
Flinn AM and Gennery AR
Orphanet Journal of Rare Diseases, 13(1), 65-65 (2018)
Regulation of MMP-9 expression by the A2b adenosine receptor and its dependency on TNF-alpha signaling
Chen H, et al.
Experimental Hematology, 39, 525-530 (2011)
Development of adenosine deaminase-specific IgY antibodies: Diagnostic and inhibitory application
Lupicka-Slowik A, et al.
Applied Biochemistry and Biotechnology, 184(4), 1358-1374 (2018)
Activation of the macrophage A2b adenosine receptor regulates tumor necrosis factor-alpha levels following vascular injury
Chen H, et al.
Experimental Hematology, 37(5), 533-538 (2009)
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