UFC8010D
Amicon® Ultra-4 Centrifugal Filter Unit, registered for IVD use
Synonym(e):
Centrifugal ultrafiltration device, 10 kDa MWCO, 4 mL sample volume
About This Item
Empfohlene Produkte
Materialien
polyethylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Qualitätsniveau
Sterilität
non-sterile
Produktlinie
Amicon®
Verpackung
pack of 24 ea (UFC801024D)
pack of 8 ea (UFC801008D)
pack of 96 ea (UFC801096D)
Hersteller/Markenname
Amicon® Ultra
Methode(n)
centrifugation: suitable (protein concentration and desalting)
protein extraction: suitable
protein purification: suitable
Länge
124 mm (4.88 in.)
Durchmesser
17.3 mm
Filtrationsfläche
3 cm2
Volumen
4 mL
Porengröße
10 kDa MWCO
Allgemeine Beschreibung
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
Anwendung
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Leistungsmerkmale und Vorteile
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Rechtliche Hinweise
Lagerklassenschlüssel
10-13 - German Storage Class 10 to 13
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