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MABF2248

Sigma-Aldrich

Anti-EBV LMP1 Antibody, clone S12

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Antikörperform

purified antibody

Klon

S12
monoclonal

Aufgereinigt durch

using protein G

Speziesreaktivität

mouse, virus

Konzentration

(Please refer to lot specific datasheet.)

Methode(n)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG2aκ

UniProt-Hinterlegungsnummer

P03230

Allgemeine Beschreibung

A fusion protein (pKH548) consisting of 189 amino acids from the C-terminus region of LMP1 coupled to beta-galactosidase.

Spezifität

Clone S12 is a mouse monoclonal antibody that specifically detects Latent membrane protein 1 (LMP1) in Epstein-Barr virus transformed cells.

Anwendung

Immunocytochemistry Analysis: A representative lot detected EBV LMP1 in Immunocytochemistry applications (Xu, J., et. al. (2002). J Virol. 76(8):4080-6).

Flow Cytometry Analysis: A representative lot detected EBV LMP1 in Flow Cytometry applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Western Blotting Analysis: A representative lot detected EBV LMP1 in Western Blotting applications (Choi, I.K., et. al. (2018). Proc Natl Acad Sci USA. 115(4):E686-E695; Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65; Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunofluorescence Analysis: A representative lot detected EBV LMP1 in Immunofluorescence applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunohistochemistry Analysis: A representative lot detected EBV LMP1 in Immunohistochemistry applications (Brink, A.A., et. al. (1997). J Clin Pathol. 50(11):911-8; Khabir, A., et. al. (2005). Virol J. 2:39).

Immunoprecipitation Analysis: A representative lot immunoprecipitated EBV LMP1 in Immunoprecipitation applications (Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65).

Qualität

Evaluated by Western Blotting in Raji cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected LMP1 in lysates from Raji cells.

Physikalische Eigenschaften

~59 kDa observed; 41.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Virus transformed human cells.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysenzertifikate (COA)

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