MAB3234
Anti-Cytokeratin 18 Antibody, clone RGE53
clone RGE53, Chemicon®, from mouse
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
RGE53, monoclonal
Speziesreaktivität
human, pig, mouse, chicken, canine, rat, rabbit, hamster
Hersteller/Markenname
Chemicon®
Methode(n)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotyp
IgG1
Eignung
not suitable for immunohistochemistry (Paraffin)
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... KRT18(3875)
Verwandte Kategorien
Allgemeine Beschreibung
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Spezifität
Specifically recognizes glandular epithelial cells from gastro-intestinal tract, respiratory tract and urogenetical tract. As well as endocrine and exocrine tissues and mesothelial cells. It is unreactive with stratified squamous epithelia (esophagus, epidermis, ectocervix). In transitional epithelium of the bladder RGE53 only reacts with the superficial (umbrella) cells. RGE53 can distinguish adenocarcinomas and mesotheliomas from squamous cell carcinoma and non-epithelial tumours. The antibody is directed against 45 kD cytokeratin protein (cytokeratin 18). Cross reactivity with; Keratin from most mammals, birds.
Immunogen
Cytoskeletal preparation from HeLa cells
Anwendung
Research Category
Zellstruktur
Zellstruktur
Research Sub Category
Cytokeratine
Cytokeratine
Anti-Cytokeratin 18 Antibody, clone RGE53 is an antibody against Cytokeratin 18 for use in FC, IC, IH & WB.
Western blot
Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.
Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.
Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on fresh frozen or acetone fixed tissue sections. Formalin fixed, paraffin embedded tissues are not recommended for RGE53.
Immunocytochemistry: (10μg/mL) Light fixation with 4% formalin, 2.5% PFA ( 5-10 minutes room temperature) or acetone. {See, Barrett, et al 2001} Permeabilization with 0.2% triton X-100 in blocking buffer if using formalin or PFA fixations.
Flow cytometry: Acetone fixed/ethanol fixed cells {See Mezosi, E et al, 2002}.
Optimal working dilutions must be determined by the end user.
Zielbeschreibung
45 kD
Verlinkung
Replaces: 04-586
Physikalische Form
Protein A purified
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Lagerung und Haltbarkeit
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hinweis zur Analyse
Control
Skin , Pancreas/tumor
Skin , Pancreas/tumor
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Produkt-Auswahlhilfe.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
DeltaNp63 is essential for epidermal commitment of embryonic stem cells.
Alain Medawar,Thierry Virolle,Philippe Rostagno,Stephanie de la Forest-Divonne et al.
Testing null
Derivation and characterization of hepatocytes from embryonic stem cells in vitro.
Shigeaki Ishizaka,Yukiteru Ouji,Masahide Yoshikawa,Kazuki Nakatani
Methods in Molecular Biology null
Sandra S Essbauer et al.
Virology journal, 8, 339-339 (2011-07-07)
Approximately 60% of emerging viruses are of zoonotic origin, with three-fourths derived from wild animals. Many of these zoonotic diseases are transmitted by rodents with important information about their reservoir dynamics and pathogenesis missing. One main reason for the gap
V Ninichuk et al.
Kidney international, 70(1), 121-129 (2006-05-26)
Multipotent mesenchymal stem or stromal cells (MSC) have shown to improve outcome of acute renal injury models, but whether MSC can delay renal failure in chronic kidney disease is not known. We injected primary MSC or saline into mice that
Noushin Amirpour et al.
Advanced biomedical research, 3, 4-4 (2014-03-05)
Retinal pigment epithelium (RPE) is a hexagonal monolayer of pigmented cells located between the neural retina and the choroid with an essential role for visual function. So, isolation, propagation and maintenance of their functional integrity of RPE are crucial for
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.