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CBL496

Sigma-Aldrich

Anti-CD34 Class II Antibody, clone QBEND/10

clone QBEND/10, Chemicon®, from mouse

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100
300

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

QBEND/10, monoclonal

Speziesreaktivität

monkey, human

Verpackung

antibody small pack of 25 μg

Hersteller/Markenname

Chemicon®

Methode(n)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

ambient

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CD34(947)

Spezifität

The antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.

KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.

FUSION PARTNER: NSO myeloma cell line

Immunogen

Human endothelial vesicles

Anwendung

Research Category
Entzündung & Immunologie
Research Sub Category
Immunoglobuline & Immunologie
Anti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P).
Identification of haemopoietic progenitor cells

Studies into the control mechanisms of early haemopoiesis

Purification of haemopoietic cells from, for example, FACS

The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts

Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.

Optimal working dilutions must be determined by the end user.

Qualität

Tested

Physikalische Form

Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Lagerung und Haltbarkeit

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Sonstige Hinweise

Please refer to lot specific datasheet.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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