Goat Anti-Human IgG Antibody, HRP conjugate

Immunoglobulin G (IgG) is the main type of glycoprotein antibody found in human serum, secreted by the B cells. It is found in blood and comprises four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains. These chains are inter-linked by di-sulfide bonds. IgG exists in four isotypes IgG1, IgG2, IgG3 and IgG4. IgG has the longest serum half-life compared to other immunoglobulins.

Specific for human IgG gamma chain. The cross-reactivities of anti-human IgG (gamma) antibody are tested in an ELISA. Minimal cross-reactivity to light chains.

The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.

ELISA: 1:3,000

Immunohistochemistry: 1:200-1:500 (1-4)

Immunocytochemistry: 1:200-1:500 (1-4)

Western Blot, chromogenic: 1:200-1-500

Western Blot, chemiluminescent: 1:2,000-1:5,000

Optimal working dilutions must be determined by the end user.

Immunoglobulin G (IgG) initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a vital role in antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associated with allergy. Maternal IgG is transferred to the fetus through the placenta that is crucial for the immune defense of the neonate against infections. Digestion of IgG by papain results in the production of fragment antigen-binding (Fab). Pepsin digestion of IgG leads to the generation of fragment crystallizable (Fc). Levels of IgG in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients can be used as a potential tool to evaluate the severity and prognosis of coronavirus disease 2019 (COVID-19).

ImmunoAffinity Purified

The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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