AP112B
Goat Anti-Human IgG Antibody, biotin conjugate
Chemicon®, from goat
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Empfohlene Produkte
Biologische Quelle
goat
Qualitätsniveau
Konjugat
biotin conjugate
Antikörperform
affinity purified immunoglobulin
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Speziesreaktivität
human
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
western blot: suitable
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Verwandte Kategorien
Allgemeine Beschreibung
IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Spezifität
The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
Immunogen
Prepared from purified human IgG.
Anwendung
Research Category
Sekundär- & Kontrollantikörper
Sekundär- & Kontrollantikörper
Research Sub Category
Sekundärantikörper gegen Gesamtimmunglobulin
Sekundärantikörper gegen Gesamtimmunglobulin
EIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.
Optimal working dilutions must be determined by the end user.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.
Optimal working dilutions must be determined by the end user.
This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.
Physikalische Form
ImmunoAffinity Purified
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
Lagerung und Haltbarkeit
Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Stefania Rosito et al.
Journal of cellular and molecular medicine, 22(2), 1236-1246 (2017-10-22)
Neuromyelitis optica (NMO) is an autoimmune demyelinating disease of the central nervous system (CNS) caused by autoantibodies (NMO-IgG) against the water channel aquaporin-4 (AQP4). Though AQP4 is also expressed outside the CNS, for example in skeletal muscle, patients with NMO
Herpes simplex virus type-2 specific glycoprotein G-2 immunomagnetically captured from HEp-2 infected tissue culture extracts.
Charles R Clavet, Aaron B Margolin, Patrick M Regan
Journal of Virological Methods null
Qun Zhou et al.
mAbs, 12(1), 1814583-1814583 (2020-09-08)
Antibodies mediate effector functions through Fcγ receptor (FcγR) interactions and complement activation, causing cytokine release, degranulation, phagocytosis, and cell death. They are often undesired for development of therapeutic antibodies where only antigen binding or neutralization would be ideal. Effector elimination
Praveen K Amancha et al.
Journal of immunology (Baltimore, Md. : 1950), 191(12), 6060-6070 (2013-11-15)
The programmed cell death-1 (PD-1)/programmed cell death ligand-1 pathway has been shown to limit cell-mediated effector functions during chronic viral infections impeding clearance of pathogens. As a strategy to reverse this exhaustion and increase T cell polyfunctionality, PD-1 ligands were
Francesco Pisani et al.
PloS one, 10(11), e0143679-e0143679 (2015-11-26)
Serological markers of Nuromyelitis Optica (NMO), an autoimmune disorder of the central nervous system, are autoantibodies targeting the astrocytic water channel aquaporin-4 (AQP4). We have previously demonstrated that the main epitopes for these autoantibodies (AQP4-IgG) are generated by the supramolecular
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