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ABE543

Sigma-Aldrich

Anti-PRDM16 Antibody

from rabbit, purified by affinity chromatography

Synonym(e):

PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

100

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

human

Speziesreaktivität (Voraussage durch Homologie)

horse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), guinea pig (based on 100% sequence homology)

Methode(n)

immunohistochemistry: suitable ((Paraffin))
immunoprecipitation (IP): suitable
western blot: suitable

NCBI-Hinterlegungsnummer

NP_071397

UniProt-Hinterlegungsnummer

Q9HAZ2

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PRDM16(63976)

Allgemeine Beschreibung

Anti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Target molecular weight ~140 kDa observed; 140.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

KLH-conjugated linear peptide corresponding to human PRDM16.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen
Research Sub Category
Chromatin-Biologie (ChIP)
Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IP & IHC.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated PRDM16 from 500 µg of K562 nuclear extract.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRDM16 in human kidney tissues.

Qualität

Evaluated by Western Blotting in LNCaP nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 10 µg of LNCaP nuclear extract.

Zielbeschreibung

~140 kDa observed. Four isoforms are known to exist. This protein has been observed at 150 kDa, 135 kDa, and 100 kDa (EMD Millipore Cat. No. ABD130). Uncharacterized band(s) may be observed in some cell lysates.

Physikalische Form

Affinity purified
Purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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