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ABE316

Sigma-Aldrich

Anti-ARID1B Antibody

from rabbit, purified by affinity chromatography

Synonym(e):

AT-rich interactive domain-containing protein 1B, ARID domain-containing protein 1B, BRG1-associated factor 250b, BAF250B, BRG1-binding protein hELD/OSA1, Osa homolog 2, hOsa2, p250R

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

100

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

human

Methode(n)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI-Hinterlegungsnummer

NP_059989

UniProt-Hinterlegungsnummer

Q8NFD5

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ARID1B(57492)

Allgemeine Beschreibung

ARID1B (AT rich interactive domain 1B), also known as BAF250B, p250R and hOsa2, is a nuclear protein and a member of the SWI/SNF-A chromatin-remodeling complex. It is involved in repression of select genes by chromatin remodeling and transcriptional activation. It non-specifically binds DNA and alters DNA nucleosome topology. The cause of mental retardation autosomal dominant type 12 (MRD12) are defects in ARID1B. High levels of ARID1B can be found in heart, skeletal muscle and kidney.

Immunogen

KLH-conjugated linear peptide corresponding to human ARID1B.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen
Research Sub Category
Chromatin-Biologie (ChIP)
Immunoprecipitation Analysis: A representative lot immunoprecipitated ARID1B from Raji cell nuclear extract.
This Anti-ARID1B Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of ARID1B.

Qualität

Evaluated by Western Blot in Raji cell nuclear extract.

Western Blot Analysis: 1.0 µg/mL of this antibody detected ARID1B in 10 µg of Raji cell nuclear extract.

Zielbeschreibung

~250 kDa observed. Uncharacterized bands may be observed at ~16 kDa and ~32 kDa in some lysates.. Uniprot describes 4 isoforms produced by alternative splicing at ~236 kDa, ~237 kDa, ~241 kDa and ~162 kDa This antibody will recognize all isoforms.

Physikalische Form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Raji cell nuclear extract

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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