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AB1284

Sigma-Aldrich

Anti-Heme Oxygenase 1 Antibody

Chemicon®, from rabbit

Synonym(e):

HO-1, HSP32

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

100

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

bovine, mouse, rat

Darf nicht reagieren mit

canine

Hersteller/Markenname

Chemicon®

Methode(n)

immunohistochemistry: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

NM_002133.1

UniProt-Hinterlegungsnummer

P09601

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

bovine ... Hmox1(513221)
dog ... Hmox1(442987)
mouse ... Hmox1(15368)
rat ... Hmox1(24451)

Spezifität

Recognizes Heme oxygenase-1.

Immunogen

A synthetic peptide with the amino acid sequence H-D-L-S-E-A-L-K-E-A-T-K-E-V-H-C-NH2 corresponding to amino acid residues [12-25] + Cys-NH2 of the human HO-1 protein (Yoshida et al., 1988).

Anwendung

Research Category
Stoffwechsel
Research Sub Category
Toxikologie & Arzneimittelresistenz
This Anti-Heme Oxygenase 1 Antibody is validated for use in IH, WB for the detection of Heme Oxygenase 1.
Western Blot: 1:1,000 (using ECL). A single band of ~32 kD is observed. Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (Catalog Number AG253). Also available are microsomal tissue preparations for control purposes (Rat spleen microsomal preparation, Catalog Number AG257).

Immunohistochemistry: 1:50-1:1,000 on human airway biopsy, human spleen, human kidney and porcine arteries.

Optimal working dilutions must be determined by the end user.

Zielbeschreibung

32 kDa

Physikalische Form

ImmunoAffinity Purified
Affinity Purified immunoglobulin. Liquid in PBS containing 0.01M sodium azide.

Lagerung und Haltbarkeit

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Lung

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Resveratrol increases vascular oxidative stress resistance.
Ungvari, Z; Orosz, Z; Rivera, A; Labinskyy, N; Xiangmin, Z; Olson, S; Podlutsky, A; Csiszar, A
American Journal of Physiology. Heart and Circulatory Physiology null
Heme oxygenase-1 could mediate the protective effects of hyperbaric oxygen preconditioning against hepatic ischemia-reperfusion injury in rats.
Yi Liu,Xue-Jun Sun,Ji Liu,Zhi-Min Kang,Xiao-Ming Deng
Clinical and Experimental Pharmacology & Physiology null
Purification and properties of heme oxygenase from pig spleen microsomes
Yoshida T, and Kikuchi G.
The Journal of Biological Chemistry, 253, 4224-4229 (1978)
Solubilization and partial purification of heme oxygenase from rat liver.
Maines, M D, et al.
The Journal of Biological Chemistry, 252, 5900-5903 (1977)
Up-regulation of heme oxygenase-1 by isoflurane preconditioning during tolerance against neuronal injury induced by oxygen glucose deprivation.
Qifang Li, Yesen Zhu, Hong Jiang, Hui Xu, Heping Liu
Acta biochimica et biophysica Sinica null
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