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Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate

Upstate®, from mouse

Synonym(e):

Anti-MPM-2 Cy5 Antibody, Cy5 Anti-MPM-2, Phospho-Ser/Thr-Pro Detection

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Konjugat

CY5 conjugate

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

monoclonal

Speziesreaktivität

vertebrates

Hersteller/Markenname

Upstate®

Methode(n)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

Isotyp

IgG1

Versandbedingung

wet ice

Posttranslationale Modifikation Target

phosphorylation (pSer/pThr)

Allgemeine Beschreibung

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Progression of cells from interphase to mitosis involves alterations in cell structures and activities. The transition from G2 to M phase is induced by M phase-promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M-phase phosphoproteins (MPPs, or MPHOSPHs) permit disassembly of interphase structures and generation of M-phase enzymatic activities and structures. Also, known as Forkhead-related protein FKHL16 or Hepatocyte nuclear factor 3 forkhead homolog 11.

Spezifität

Recognizes a phosphorylated epitope (phospho-[Ser/Thr]Pro) found in phosphoproteins such as MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type.

Immunogen

Mitotic human HeLa cell lysate

Anwendung

Research Category
Epigenetik & nukleäre Funktionen
Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, Cy5 conjugate is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in IF, WB, ICC.

Qualität

Routinely evaluated by immunoblot on colcemid-treated HeLa cell lysates.

Physikalische Form

Protein G Purified
100μg Cy5-conjugated mouse IgG1 in 200μl of PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide.

Lagerung und Haltbarkeit

1 year at 4°C from date of shipment

Hinweis zur Analyse

Control
Colcemid-treated HeLa cell lysates

Rechtliche Hinweise

TWEEN is a registered trademark of Croda International PLC
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Thomas Blasi et al.
Nature communications, 7, 10256-10256 (2016-01-08)
Imaging flow cytometry combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. Here we demonstrate label-free prediction of DNA content and quantification of the mitotic cell cycle phases by applying supervised machine learning to morphological features extracted from
Nicola Brownlow et al.
Nature communications, 5, 5685-5685 (2014-12-09)
Exit from mitosis is controlled by silencing of the spindle assembly checkpoint (SAC). It is important that preceding exit, all sister chromatid pairs are correctly bioriented, and that residual catenation is resolved, permitting complete sister chromatid separation in the ensuing

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