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Merck

810253P

Avanti

dehydroergosterol (DHE)

ergosta-5,7,9(11),22-tetraen-3β-ol, powder

Synonym(e):

DHE

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About This Item

Empirische Formel (Hill-System):
C28H42O
CAS-Nummer:
Molekulargewicht:
394.63
UNSPSC-Code:
12352211
NACRES:
NA.25

Assay

>99% (TLC)

Form

powder

Verpackung

pkg of 1 × 1 mg (with stopper and crimp cap (810253P-1mg))

Hersteller/Markenname

Avanti Polar Lipids 810253P

Versandbedingung

dry ice

Lagertemp.

−70°C

InChI

1S/C28H42O/c1-18(2)19(3)7-8-20(4)24-11-12-25-23-10-9-21-17-22(29)13-15-27(21,5)26(23)14-16-28(24,25)6/h7-10,14,18-20,22,24-25,29H,11-13,15-17H2,1-6H3/b8-7+/t19-,20+,22-,24+,25-,27-,28+/m0/s1

InChIKey

QSVJYFLQYMVBDR-CMNOFMQQSA-N

Verwandte Kategorien

Allgemeine Beschreibung

Dehydroergosterol (DHE) is a fluorescent sterol that possesses similar biophysical properties as cholesterol. It is found in yeast cells and other fungi.

Anwendung

Dehydroergosterol (DHE) is suitable for the transfer of DHE from a population of donor (LD) to acceptor (LA) liposomes.

Biochem./physiol. Wirkung

Dehydroergosterol (DHE) is used for the visualization of intracellular traffic and intracellular transport kinetics.

Verpackung

2 mL Amber Serum Vial with Stopper and Crimp Cap (810253P-1mg)

Rechtliche Hinweise

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Reynold Homan et al.
Analytical biochemistry, 441(1), 80-86 (2013-07-16)
We describe a simple but sensitive fluorescence method to accurately detect the esterification activity of lecithin:cholesterol acyltransferase (LCAT). The new assay protocol employs a convenient mix, incubate, and measure scheme. This is possible by using the fluorescent sterol dehydroergosterol (DHE)
Molecular basis for sterol transport by StART-like lipid transfer domains
Horenkamp FA, et al.
The Embo Journal, 37(6) (2018)
Mohsen Pourmousa et al.
The journal of physical chemistry. B, 118(26), 7345-7357 (2014-06-04)
Although dehydroergosterol (DHE) is one of the most commonly used cholesterol (CHOL) reporters, it has remained unclear why it performs well compared with most other CHOL analogues and what its possible limitations are. We present a comprehensive study of the
Daniel Wüstner et al.
Microscopy research and technique, 74(1), 92-108 (2010-12-25)
Dehydroergosterol (DHE) is an intrinsically fluorescent sterol with absorption/emission in the ultraviolet (UV) region and biophysical properties similar to those of cholesterol. We compared the potential of UV-sensitive low-light-level wide-field (UV-WF) imaging with that of multiphoton (MP) excitation microscopy to
Olav Garvik et al.
Chemistry and physics of lipids, 159(2), 114-118 (2009-05-30)
The fluorescent sterol dehydroergosterol (DHE) is often used as a marker for cholesterol in cellular studies. We show by vesicle fluctuation analysis that DHE has a lower ability than cholesterol to stiffen lipid bilayers suggesting less efficient packing with phospholipid

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