WHAWB120306
QIAcard™ Indicating FTA™ Cards
classic, 4 sample areas per card, pkg of 25 cards
Synonym(e):
QIAcard™ FTA™ Indicating Classic (25), Whatman FTA, Z747491
About This Item
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Beschreibung
Indicating FTA Classic Card with 4 sample areas per card, 125 μl maximum volume/sample area, 500 μl maximum total volume/card
Hersteller/Markenname
Qiagen WB120306
Verpackung
pkg of 25 cards
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Allgemeine Beschreibung
QIAcard™ FTA™ classic format with 4 spot area to collect, stabilize, process, transport, and archive colored samples such as blood. FTA™ technology enables cell lysis on contact, denatures proteins and immediate stabilization and protection of nucleic acids.
FTA™ classic cards utilize QIAcard™ FTA™ technology that simplifies the handling and processing of nucleic acids. FTA™ Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidation and UV damage. FTA™ cards rapidly inactivate organisms, including blood-borne pathogens, and prevent the growth of bacteria and other microorganisms.
Convenient for protocols that require different locations for testing and archiving samples. Different samples can be processed independently.
Leistungsmerkmale und Vorteile
- Includes a pink dye that turns white when a colorless sample is applied.
- 4 sample areas for application of up to 500 μl sample volume per card.
- Simply apply your sample to the FTA™ Card. Cell membranes and organelles are lysed and the released nucleic acids are entrapped in the fibers of the matrix. The nucleic acids remain immobilized and are preserved for transport, immediate processing or long-term room temperature storage.
- Since captured nucleic acids are preserved, FTA™ Cards facilitate sample collection in remote locations and simplify sample transport.
- Ship your samples back to the laboratory without expensive special handling or dry ice and process at your convenience.
- FTA™ Cards can be used with virtually any sample type: Blood, Cultured cells, Buccal cells, Plasmids, and Solid tissue.
- Captured nucleic acids are ready for purification when you are. Just take a punch from the FTA™ Card, wash with FTA™ Purification Reagent and rinse with TE-1 (10 mM Tris-HCI, 0.1 mM EDTA, pH 8) buffer. DNA on the washed punch is ready to use in applications such as PCR, SNP analysis, and real-time PCR. Since PCR products remain in solution, the punch can be used for multiple amplifications.
Sonstige Hinweise
Field of Use : For internal research use only
Rechtliche Hinweise
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Artikel
This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.
Description of common problems that can occur during genomic DNA preparation
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
Protokolle
This page shows genomic DNA purification from sample on FTA elute from Cytiva.
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
A number of options are available for sample collection, transport, archiving, and DNA purification.
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
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