- Dynamic combinatorial mass spectrometry leads to metallo-beta-lactamase inhibitors.
Dynamic combinatorial mass spectrometry leads to metallo-beta-lactamase inhibitors.
Journal of medicinal chemistry (2008-01-22)
Benoît M R Liénard, Rebekka Hüting, Patricia Lassaux, Moreno Galleni, Jean-Marie Frère, Christopher J Schofield
PMID18205296
ABSTRACT
The use of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial library of thiols/disulfides with the BcII metallo-beta-lactamase enabled the rapid identification of an inhibitor with a K(i) of < 1 microM. The study exemplifies the utility of protein-MS for screening dynamic mixtures of potential enzyme-inhibitors.