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  • YihE kinase is a central regulator of programmed cell death in bacteria.

YihE kinase is a central regulator of programmed cell death in bacteria.

Cell reports (2013-02-19)
Angella Dorsey-Oresto, Tao Lu, Michael Mosel, Xiuhong Wang, Tal Salz, Karl Drlica, Xilin Zhao
ABSTRACT

Stress-mediated programmed cell death (PCD) in bacteria has recently attracted attention, largely because it raises novel possibilities for controlling pathogens. How PCD in bacteria is regulated to avoid population extinction due to transient, moderate stress remains a central question. Here, we report that the YihE protein kinase is a key regulator that protects Escherichia coli from antimicrobial and environmental stressors by antagonizing the MazEF toxin-antitoxin module. YihE was linked to a reactive oxygen species (ROS) cascade, and a deficiency of yihE stimulated stress-induced PCD even after stress dissipated. YihE was partially regulated by the Cpx envelope stress-response system, which, along with MazF toxin and superoxide, has both protective and destructive roles that help bacteria make a live-or-die decision in response to stress. YihE probably acts early in the stress response to limit self-sustaining ROS production and PCD. Inhibition of YihE may provide a way of enhancing antimicrobial lethality and attenuating virulence.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nalidixic acid sodium salt, powder
Sigma-Aldrich
Nalidixic acid, ≥98%
Supelco
Nalidixic acid, analytical standard