Iron (Total) in Plant Material

Reagents

Product No. 173016 Spectroquant^® VIS Spectrophotometer Prove 100 or
Product No. 173017 Spectroquant^® UV/VIS Spectrophotometer Prove 300 or
Product No. 173018 Spectroquant^® UV/VIS Spectrophotometer Prove 600 or
Product No. 109748 Spectroquant^® Photometer NOVA 30 or
Product No. 109751 Spectroquant^® Photometer NOVA 60 or
Product No. 173632 Spectroquant^® Colorimeter Move 100
Product No. 114946 Rectangular cells 10 mm and/or
Product No. 114947 Rectangular cells 20 mm and/or
Product No. 114944 Rectangular cells 50 mm
Product No. 114549 Spectroquant^® Iron Cell Test
Product No. 114896 Spectroquant^® Iron Cell Test*
Product No. 114761 Spectroquant^® Iron Test
Product No. 100796 Spectroquant^® Iron Test
Product No. 100456 Nitric acid 65 % for analysis
Product No. 100731 Sulfuric acid 95 - 97 % for analysis
Product No. 100519 Perchloric acid 70 % for analysis
Product No. 116754 Water for analysis

*not compatible with Move 100

Sample Preparation

Ground the plant material finely and pass through a sieve (mesh 0.4 - 0.7 mm) and dry the material at 105 °C for 3 hours.

Add 1.0 g of the dried material to a mixture of 1.5 mL nitric acid 65 %, 0.5 mL perchloric acid 70 %, 1 mL sulfuric acid 95 - 97 % and heat until white fumes are envolved (fume cupboard!). Dissolve the residue, after cooling, in water for analysis and fill up to the mark in a 100-mL standard volumetric flask and mix well.

Analysis

Determine with the above mentioned test kits.

Calculation