- Small molecule-triggered Cas9 protein with improved genome-editing specificity.
Small molecule-triggered Cas9 protein with improved genome-editing specificity.
Nature chemical biology (2015-04-08)
Kevin M Davis, Vikram Pattanayak, David B Thompson, John A Zuris, David R Liu
PMID25848930
ZUSAMMENFASSUNG
Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the presence of a cell-permeable small molecule by inserting an evolved 4-hydroxytamoxifen-responsive intein at specific positions in Cas9. In human cells, conditionally active Cas9s modify target genomic sites with up to 25-fold higher specificity than wild-type Cas9.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Monoklonaler ANTI-FLAG® M2-Antikörper in Maus hergestellte Antikörper, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)